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vectashield mounting medium with dapi  (Vector Laboratories)
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recombinant proteins dapi  (Thermo Fisher)
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Thermo Fisher recombinant proteins dapi
KEY RESOURCES TABLE
Recombinant Proteins Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield anti fade medium  (Vector Laboratories)
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KEY RESOURCES TABLE
Vectashield Anti Fade Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield anti fade medium with dapi  (Vector Laboratories)
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Vectashield Anti Fade Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rrid ab 2336788 software  (Vector Laboratories)
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Rrid Ab 2336788 Software, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield hardset antifade mounting medium with dapi  (Vector Laboratories)
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Vectashield Hardset Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining <t>DAPI</t> was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa
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4 6 diamidino 2 phenylindole dapi  (Vector Laboratories)
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The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining <t>DAPI</t> was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa
4 6 Diamidino 2 Phenylindole Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4, 6-diamidino-2-phenylindole vectashield®+dapi  (AbCys s a)
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The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining <t>DAPI</t> was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa
4, 6 Diamidino 2 Phenylindole Vectashield®+Dapi, supplied by AbCys s a, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield anti fade mounting medium with dapi  (Novus Biologicals)
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Novus Biologicals vectashield anti fade mounting medium with dapi
The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining <t>DAPI</t> was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa
Vectashield Anti Fade Mounting Medium With Dapi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi  (Vector Laboratories)
96
Vector Laboratories dapi
The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining <t>DAPI</t> was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa
Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti fade dapi solution  (Vector Laboratories)
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Vector Laboratories anti fade dapi solution
The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining <t>DAPI</t> was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa
Anti Fade Dapi Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Current biology : CB

Article Title: Homeostatic plasticity shapes the retinal response to photoreceptor degeneration

doi: 10.1016/j.cub.2020.03.033

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-ChAT Abcam RRID:AB_2079595 Mouse anti-PKCα Sigma RRID:AB_477375 Mouse anti-Gαo Millipore RRID:AB_94671 Mouse anti-Syt2 Zebrafish International Resource Center RRID:AB_10013783 Chicken anti-GFP ThermoFisher RRID:AB_2534023 Rabbit anti-DsRed BD Biosciences RRID:AB_394264 Mouse anti-RFP Abcam RRID:AB_1141717 Mouse anti-Gpr179 Millipore RRID:AB_2069582 Rabbit anti-cone arrestin Millipore RRID:AB_1163387 Rabbit anti-M-opsin Millipore RRID:AB_177456 Goat anti-S-opsin Santa Cruz RRID:AB_2158332 Sheep anti-mGluR6 [ 58 ] N/A Mouse anti-CtBP2 BD Biosciences RRID:AB_399431 Goat ant-rabbit IgG DyLight 405 ThermoFisher RRID:AB_1965945 Donkey anti-chicken IgY Alexa 488 ThermoFisher RRID:AB_2534096 Goat anti-mouse IgG Alexa 488 ThermoFisher RRID:AB_2534084 Donkey ant-rabbit IgG Alexa 488 ThermoFisher RRID:AB_2556546 Donkey anti-rabbit IgG Alexa 568 ThermoFisher RRID:AB_2534017 Donkey anti-mouse IgG Alexa 568 ThermoFisher RRID:AB_2534013 Donkey anti-goat IgG Alexa 633 ThermoFisher RRID:AB_2535739 Goat anti-mouse IgG Alexa 633 ThermoFisher RRID:AB_2535768 Donkey anti-sheep IgG Alexa 633 ThermoFisher RRID:AB_2535754 Bacterial and Virus Strains AAV-Grm6-YFP [ 4 ] N/A AAV-Grm6-tdTomato [ 5 ] N/A Chemicals, Peptides, and Recombinant Proteins DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher RRID:AB_2629482 Vectashield medium Vector Laboratories RRID:AB_2336789 Diphtheria toxin Sigma Cat# D0564 Experimental Models: Organisms/Strains Wild-type (C57Bl6/J) Jackson Laboratory RRID:IMSR_JAX:000664 B6;Tg(OPN1LW-cre)4Yzl (HRGP-Cre) Jackson Laboratory RRID:IMSR_JAX:032911 B6; Gt(ROSA)26Sortm1(HBEGF)Awai (iDTR) Jackson Laboratory RRID:IMSR_JAX:007900 Grm6-tdTomato [ 34 ] N/A Software and Algorithms MATLAB The Mathworks RRID:SCR_001622 LKC LKC Technologies www.lkc.com ISCAN ISCAN Inc. www.iscaninc.com Fiji [ 59 ] RRID:SCR_002285 Amira ThermoFisher RRID:SCR_014305 Open in a separate window KEY RESOURCES TABLE Cone degeneration elicits homeostatic rewiring of bipolar cell dendrites Homeostatic plasticity differs between bipolar cell types Homeostatic plasticity of bipolar cells declines steeply with age Homeostatic plasticity of bipolar cells determines visual function after cone loss

Techniques: Virus, Recombinant, Plasmid Preparation, Software

The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining DAPI was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Soluble polysialylated NCAM: a novel player of the innate immune system in the lung

doi: 10.1007/s00018-013-1342-0

Figure Lengend Snippet: The secretion of intracellularly localized polySia-NCAM of human lung epithelial cell line A549 is induced by IL-1β and LPS. a Western-blot analyses of cell lysates of human A549 cells were performed using a mAb against polySia and NCAM (123C3) before and after endoN treatment. b, c Cultured A549 cells were fixed with methanol and stained with fluorescently labeled inactive endoN and b anti-NCAM mAb 123C3 or c polyclonal antibodies to visualize the trans-Golgi apparatus (anti-TGN38). For nuclear staining DAPI was used. d In addition, polySia was visualized in cells after stimulation with IL-1β (200 ng/ml) or LPS (100 ng/ml). Scale bars for c and d = 25 μm. e, f Polysialylated proteins were purified from supernatants of differently treated A549 cells and visualized by Western blotting using an anti-polySia mAb and after depolysialylation with a mAb against NCAM (123C3). g In an analogous cell lysates of IL-1β-treated cells were analyzed. h Western blots against polySia and NCAM (after depolysialylation) were performed using supernatants of untreated A549 cells as well LPS-stimulated cells in the absence or presence of TAPI-2. Apparent molecular masses of standard proteins are indicated in kDa

Article Snippet: For immunostaining against polySia, NCAM, and TGN38, cells were fixed with methanol at −20 °C for 10 min, blocked with 1 % BSA in PBS for 15 min, and incubation with appropriately diluted primary antibodies or biotinylated inactive endoN was continued for 1 h. After three washing steps with PBS and exposure to streptavidin–FITC conjugate and an anti-mouse-Rhodamine secondary antibody (Dianova, Hamburg, Germany), the final mounting VECTASHIELD with DAPI (Linaris, Munich, Germany) was added to the fixed cells.

Techniques: Western Blot, Cell Culture, Staining, Labeling, Purification